It is proposed to carry out embedded Electronic Systems (ES) for the supervision and characterization of cells and cell cultures, which allow to act on them by means of programmable electrostimulation signals.
The objective is to study, know and improve the techniques of cell differentiation towards different types of lineages and tissues of interest in regenerative medicine. The design and manufacture of systems with reduced weight and size, energy autonomous and wireless are pursued, which reduce the workload, automate experiments and monitor in real time the evolution of a cell culture based on the electrical BioImpedance (BI) as a marker. . It is proposed to monitor the evolution of cell lines: neuroblastomas, myoblastomas and osteoblasts, useful in neuronal therapies and engineering of muscle and bone tissues, towards the conformation of the corresponding cell or tissue type, optimizing the differentiation processes through the adequate design of signals of electrical stimulation. From the results obtained in a first measurement setup, two more setups are proposed: one oriented to the clinical development of tissue engineering; and another dedicated to the study and characterization at the cellular level of electrostimulation processes, through the manufacture of microelectrode arrays (MicroElectrode Array, MEA). Taking advantage of this last setup, cell motility experiments are proposed to determine the position and velocity of tumor cells (MCF7) in cultures, and their use in cancer studies. In summary, ES will be developed for monitoring and electrostimulation measuring electrical BIs, in parallel to a cell and tissue biometry procedure for the real-time identification of the biological material differentiated or not, and its dynamic characteristics: position, trajectory and speed.
The results will be validated using biomedical experimentation standards in the proposed cell lines.